Amicon ultra 4 manual

 · In short, DNA extract and 1 × TEbuffer were added to the devices giving a total volume of 2 mL for Amicon Ultra 30 K and 4 mL for Microsep 30 K, followed by centrifugation ( × g for 10 minutes for Amicon Ultra 30 K and × g for 15 minutes for Microsep 30 K). Concentration and percent recovery using Amicon® Ultra Filters: 4 different devices (Amicon® Ultra mL, Amicon® Ultra-2 mL, Amicon® Ultra-4 mL, Amicon® Ultra mL), were tested with four different proteins (3 kDa Cytochrome C, 10 kDa Cytochrome C, 30 kDa BSA and kDa IgG) to determine percent recovery and concentration www.doorway.rug: manual. Any rotor capable of accommodating cm in diameter and mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and mm in length for the Amicon Ultra can be used for Amicon Ultra units. What is the chemical compatibility of Amicon Ultra? Amicon Ultra units are intended for use with biological fluids and aqueous Missing: manual.

We’ve engineered Amicon ® Ultra filters with low-binding Ultracel ® regenerated cellulose membranes to ensure more precise molecular weight cut-offs. Amicon ® Ultra centrifugal filters are available in four different volumes: Amicon ® Ultra mL Centrifugal Filters. Amicon ® Ultra 2 mL Centrifugal Filters. Amicon Ultra-4 Amicon Ultra Centrifugal Filter Devices Concentration Factor (X) Spin Time (min) 0 20 10 15 30 40 60 80 Spin Time vs Concentration Factor Performance shows results in Amicon Ultra 4 mL device with a 4 mL starting sample of cytochrome c, 12, daltons ( mg/mL) at 3 spin times (10, 15, and 30 minutes). Filtrate. Amicon Ultra units are available in mL, 4mL 15ml volumes. What is the recommended rotor for Amicon Ultra units? Any rotor capable of accommodating cm in diameter and mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and mm in length for the Amicon Ultra can be used for Amicon Ultra units.

4. Introduction. The OriGene IgG biotinylation kit utilizes a novel Up to 4 mL Amicon Ultra-4 Centrifugal Filter Unit with Ultracel membrane. Centrifuge device at 4, × g for 15 minutes in a swinging-bucket rotor. 3. Recover the labeled antibody from the Amicon® Ultra device by reverse spin. 4. The protocols in this User Manual require the handling of adeno-associated 4. 6. Amicon Ultra, kDa. 2. III. Storage. Concentrating solution B. 4℃.